Basic electron microscopy in transmission

Diagnostic Transmission Electron Microscopy

The high resolution and sensitivity of electron microscopy is still a valuable tool, and for some diseases it is the gold standard in pathological diagnosis. Today the sample turnaround time for processing in the lab can be significantly reduced from days to hours by the microwave technology. In urgent clinical cases, microwaveassisted tissue processing, in combination with digital image acquis...

Conventional transmission electron microscopy

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during ex...

Electron Diffraction Using Transmission Electron Microscopy

Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the strong atomic scattering, and the ability to examine tiny volumes of matter (≈1...

Energy-Filtering Transmission Electron Microscopy

Holography and transmission electron microscopy

The basic principles and methods of off-axis electron holography are presented and illustrated by means of three examples related to its application in high resolution electron microscopy and the investigation of electric and magnetic fields in thin specimens.